Jacob, together with his older brother, Esau, was born to Isaac and Rebekah after 20 years of marriage, when his father was 60, and Abraham was 160 years old. He and Esau were markedly different in appearance and behaviour. Esau was a ruddy hunter, while Jacob was a gentle man who "dwelled in tents". 

During Rebekah's pregnancy, "the children struggled together within her".

Esau was the firstborn. His brother Jacob was born immediately afterwards, and was grasping Esau's heel. His name, Ya'akov (יעקב), derives from the Hebrew root "עקב," "heel." Commentators explain that Jacob was trying to hold Esau back from being the firstborn, and in that way claim the Abrahamic legacy for himself. According to the text, Jacob was favored by his mother, while Esau was favored by his father.  

During their youth, the twins were raised in the same environment and exposed to the same teachings of their father Isaac and grandfather Abraham. One day, Esau returned from the field faint from hunger. Seizing an opportunity, Jacob informed Esau that he would sell him some lentil soup which he had just cooked, in exchange for the birthright which belonged to Esau as the older brother. Esau agreed "I am going to die — what is this birthright to me?" 

When Isaac was aged and blind, he decided to bless his eldest son before he died. He sent Esau out in the fields to hunt down some meat and prepare him a meal, after which he would receive his blessing. 

Rebecca overheard this exchange. As Esau went out to the hunt, she instructed Jacob to fetch her two goats so that she could prepare a tasty meal for his father, and commanded him to bring the meal to Isaac to receive the blessing in his brother's stead. Jacob protested that his father might notice the substitution through touch, since Esau was hairy and he was smooth-skinned. Rebecca told him not to worry, and placed hairy goatskins over his neck and arms.

Thus disguised, Jacob went into his father's tent. Isaac was surprised that he had returned so soon from the "hunt." "Who are you, my son?" Isaac asked suspiciously. "I am Esau your firstborn," Jacob replied (the Hebrew words, however, can be divided into two statements: "I" and "Esau is your firstborn"). Isaac was still suspicious and asked to feel him, since Esau was hairy. The goatskins seemed to fool him, although he maintained, "The voice is the voice of Jacob, but the hands are the hands of Esau." Nevertheless, Isaac blessed him and sent him on his way.

As soon as Jacob left the tent, Esau arrived and exposed the deception. Isaac was shaken, but he affirmed that Jacob would indeed be blessed. To Esau's pathetic entreaties, he agreed to give Esau a lesser blessing. Esau exclaimed, "Is that why he is called Jacob (יעקב), because he has deceived me (ויעקבני) these two times?" (Genesis 27:35), another play on Jacob's name. Then Esau swore to himself that he would kill Jacob in revenge as soon as his father was dead.